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目的:研究单次给予柴胡总皂苷致小鼠肝毒性损伤的机制及其时毒、量毒关系。方法:按小鼠急性肝毒性“量-时-毒”关系研究方法进行,“时-毒”关系研究:小鼠灌胃一定剂量(36.075 g/kg)的柴胡总皂苷提取物,分别于给药后不同时间点检测血清谷胱甘肽S转移酶(GST)、乳酸脱氢酶(LDH)水平;“量-毒”关系研究:给小鼠灌胃不同剂量(36.075、21.650、12.975、7.925、4.675 g/kg)的柴胡总皂苷提取物,于给药后2 h检测血清谷胱甘肽S转移酶(GST)、乳酸脱氢酶(LDH)水平。结果:对柴胡总皂苷致小鼠肝毒性“时-毒”关系研究显示:小鼠单次灌胃柴胡总皂苷提取物后1 h血清GST、LDH水平开始升高,与0 h组比较有极显著性差异;血清GST、LDH水平均于药后12 h达到峰值,之后逐渐恢复。对柴胡总皂苷致小鼠肝毒性“量-毒”关系研究显示:小鼠单次灌胃不同剂量的柴胡总皂苷提取物后2h血清GST、LDH水平均有不同程度升高,且呈现一定的剂量相关性。结论:小鼠单次灌服一定剂量柴胡总皂苷提取物可造成急性肝损伤,并呈现一定的时毒、量毒关系;影响肝脏能量代谢是柴胡总皂苷致小鼠肝毒性损伤的途径之一。
Objective: To study the mechanism of hepatotoxic injury induced by single administration of Bupleurum saponin in mice and the relationship between the time and toxic dose. Methods: According to the research method of acute hepatotoxicity in mice, the relationship between “quantity - time - toxicity” and “time - toxicity” were studied: the mice were given gavage by a certain dose (36.075 g / kg) The levels of serum glutathione S-transferase (GST) and lactate dehydrogenase (LDH) were measured at different time points after administration. The study of the relationship between “quantity-toxicity” 36.075, 21.650, 12.975, 7.925 and 4.675 g / kg, respectively. Serum glutathione S transferase (GST) and lactate dehydrogenase (LDH) levels were measured 2 h after administration. Results: The study on the relationship between time-toxicity and toxic effect of saikosaponin in mice showed that serum GST and LDH levels increased at 1 h after single intragastric administration of Bupleurum sativum total saponin, There was a significant difference between the two groups. Serum GST and LDH levels peaked at 12 h after treatment and gradually recovered. The study of the relationship between the toxic effects of Bupleurum saponin and the mice on hepatotoxicity showed that the GST and LDH levels in serum of mice were increased to different extents 2 h after single intragastric administration of different doses of Bupleurum chinense extract, And showed a certain dose-related. Conclusion: A single dose of Bupleurum chinense saponin extract can cause acute liver injury in mice with certain dose and time-dependent toxicity. The effect of hepatic energy metabolism on the hepatic toxicity of Bupleurum saponin in mice one.