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目的:①检测心力衰竭(心衰)患者血浆中IC53基因自身抗体水平;②寻找IC53自身抗体与心功能、不同病因等因素的相关性。方法:①观察组:188例,为山东莱芜市人民医院2006-06-2008-06符合心衰诊断标准的患者;对照组:160例,为在莱芜市人民医院行健康体检的健康人。②所有入选者均排除内分泌性、自身免疫性及感染性疾病,采集血样前3d避免服用免疫抑制剂、β-受体阻滞剂、血管紧张素转化酶抑制剂,入选后予心脏X线检查和超声心动图检查。③以合成肽作为抗原,首先应用酶联免疫吸附测定技术,检测348例受试者血浆中IC53自身抗体;随后选取实验结果阳性的标本为样品侧,BSA为对照侧,用Wester blot检测血浆中的抗体表达。结果:①心衰组IC53自身抗体的阳性率为21.3%(40/188),明显高于对照组5%(8/160);不同病因患者IC53自身抗体的阳性率差异无显著性;心功能Ⅳ级的阳性率明显高于心功能Ⅲ、Ⅱ级的阳性率。②IC53自身抗体在相同心功能分级不同性别之间的阳性率有显著性差异,女性明显高于男性。③Western blot结果:样品侧和对照侧蛋白表达有差异。结论:IC53自身抗体存在于多种心脏病患者的血浆中,可能与心衰时心肌结构变化和功能下降有关,与心脏病病因无明显因果关系。
PURPOSE: To detect the level of autoantibodies against IC53 in plasma of patients with heart failure (CHF). To find out the correlation between IC50 autoantibodies and cardiac function and different etiologies. Methods: ① The observation group: 188 cases were from Laiwu People’s Hospital of Shandong Province 2006-06-2008-06 in line with the diagnostic criteria of heart failure; control group: 160 cases, healthy people in Laiwu City People’s Hospital for physical examination. ②All the candidates were excluded endocrine, autoimmune and infectious diseases, collecting blood samples before 3d to avoid taking immunosuppressive agents, β-blockers, angiotensin converting enzyme inhibitors, were selected for cardiac X-ray examination And echocardiography. (3) Using synthetic peptide as antigen, we first detected the IC50 autoantibodies in plasma of 348 subjects by enzyme-linked immunosorbent assay (ELISA); then the positive samples were selected as the sample side and the BSA as the control side, Antibody expression. Results: ①The positive rate of IC53 autoantibodies in heart failure group was 21.3% (40/188), which was significantly higher than that in control group (8/160). There was no significant difference in the positive rate of IC53 autoantibodies in different etiologies. Ⅳ grade positive rate was significantly higher than the heart function Ⅲ, Ⅱ grade positive rate. ② The positive rate of IC53 autoantibodies between different sexes with the same cardiac function grading was significantly different, the female was significantly higher than the male. ③Western blot results: the protein expression of the sample side and the control side are different. Conclusion: The existence of IC53 autoantibodies in the plasma of many kinds of heart disease patients may be related to the changes of myocardial structure and function decline in heart failure, and has no obvious causal relationship with the cause of heart disease.