作者采用淋巴细胞和巨噬细胞(M )体外混合培养的方法研究日本血吸虫病人的免疫调节机制。研究对象为流行地区的8例曾患血吸虫病的农民。这些患者曾经粪检、皮试和环卵试验(Cop)检查确诊为日本血吸虫病。其中2例在10多年前用锑剂治疗过,但在近10年无1人粪检阳性。用Ficoll-Conray梯度离心法分离外周血淋巴细胞。将上述分离所得淋巴细胞倒入塑料平皿,放37℃,CO_2孵箱中60分钟后, The authors studied the immunomodulatory mechanism of Schistosoma japonicum in a mixed culture of lymphocytes and macrophages (M) in vitro. The subjects studied were 8 peasants who had schistosomiasis in endemic areas. These patients have been diagnosed as schistosomiasis japonica by fecal examination, skin test and egg ring test (Cop). Two of them were treated with antimony agents more than 10 years ago, but none of them was positive in the past 10 years. Peripheral blood lymphocytes were isolated by Ficoll-Conray gradient centrifugation. The above isolated lymphocytes were poured into plastic dishes, put 37 ℃, CO_2 incubator for 60 minutes,