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目的:通过观察大鼠追赶肥胖过程中瘦素及游离瘦素受体水平变化,进一步揭示追赶肥胖发生机制。方法:5周龄Wistar雄性大鼠适应性喂养1周后随机分为对照组(NC)和追赶肥胖组(RN),RN组予半量饮食喂养4周后开放饮食8周,NC组开放饮食12周。分别于实验第0,4,6,8,12周采集血液并剥离内脏脂肪,测内脏脂肪(以两侧肾周及附睾脂肪为代表)重量/体重比值;用ELISA法测血清游离瘦素受体(sLR)及瘦素水平。结果:实验第4周,与NC组比较,RN组血清瘦素水平、内脏脂肪/体重比值明显降低(P<0.05),sLR变化无统计学意义,开放喂养后RN组血清瘦素水平、内脏脂肪/体重比值呈时间依赖性升高,于第8周时显著高于NC组(P<0.05);而sLR在第6周时则明显低于NC组,具有统计学意义(P<0.05)。结论:在追赶生长过程中sLR浓度下降、瘦素水平增高引发瘦素抵抗,瘦素功能不足可能是造成追赶肥胖的原因。
OBJECTIVE: To observe the changes of leptin and free leptin receptor during obesity in rats to further reveal the mechanism of catching up obesity. Methods: Five weeks Wistar male rats were randomly divided into control group (NC) and catch-up obesity group (RN) one week after adaptive feeding. RN group were fed with half-dose diet for 4 weeks and then opened for 8 weeks. week. The blood was collected at the 0th, 4th, 6th, 8th and 12th week of the experiment and the visceral fat was peeled off. The weight / body weight ratio of visceral fat (represented by perirenal and epididymal fat on both sides) was measured. Body (sLR) and leptin levels. Results: Compared with NC group, serum leptin level and visceral fat / body weight ratio decreased significantly in RN group (P <0.05), and sLR change had no statistical significance in the 4th week. Serum leptin level, The fat / body weight ratio increased in a time-dependent manner, which was significantly higher than that in NC group at the 8th week (P <0.05). The sLR at 6th week was significantly lower than that in NC group (P <0.05) . CONCLUSIONS: The concentration of sLR decreased during catch-up growth and increased leptin levels led to leptin resistance. Leptin deficiency may be responsible for catching up with obesity.