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收集２４例ＰＧＬ石蜡标本，利用ＰＣＲ技术扩增ＣＤＲ－Ⅲ区，检测其免疫球蛋白重链（ＩｇＨ）基因重排，同时收集结内非霍奇金氏淋巴瘤（ＮＨＬ）、反应性增生淋巴结组织等标本作为对照。结果显示，８３％（２０／２４）Ｂ细胞性ＰＧＬ获得阳性克隆性条带７５％（２４／３２）结内Ｂ－ＮＨＬ获得阳性条带。研究表明应用ＩｇＨ基固引物进行ＰＣＲ扩增可用于ＮＨＬ的诊断和分型，证明Ｂ细胞ＰＧＬ与Ｂ－ＮＨＬ的ＩｇＨ基因重排一致。
Twenty-four PGL paraffin specimens were collected and PCR was used to amplify the CDR-III region to detect the rearrangement of its immunoglobulin heavy chain (IgH) gene. At the same time, intranodal non-Hodgkin’s lymphoma (NHL) and reactive hyperplasia lymph nodes were collected. Tissue and other specimens were used as controls. The results showed that 83% (20/24) of B-cell PGL obtained a positive clonal band with 75% (24/32) of intra-knot B-NHL to obtain a positive band. Studies have shown that the use of IgH primers for PCR amplification can be used for the diagnosis and typing of NHL, demonstrating that B cell PGL and B-NHL IgH gene rearrangement is consistent.