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目的：比较唾液酸单体间的生物活性和揭示唾液酸与吗丙嗪间的关系。方法：以兔红细胞膜上钙调蛋白作为手段。结果：Ｎ乙酰神经氨酸（５ＡｃＮｅｕ，＞２００μｍｏｌ／Ｌ）和Ｎ葡萄糖代神经氨酸（ＮｅｕＧｃ，＞５０μｍｏｌ／Ｌ）能在体外显著抑制兔红细胞膜钙调蛋白（ＣａＭ）活性（Ｐ＜０．０５）。抑制率与所用浓度基本呈对数线性。ＮｅｕＧｃ的抑制强度约为５ＡｃＮｅｕ的２～４倍。此外５ＡｃＮｅｕ（２００μｍｏｌ／Ｌ）能使低浓度的吗丙嗪（２５～１００μｍｏｌ／Ｌ）增加对ＣａＭ的抑制率，因为在这一浓度范围内，吗丙嗪不能显示抗兔红细胞ＣａＭ的活性。结论：本工作进一步证明唾液酸单体具抗细胞钙调蛋白作用。吗丙嗪可能通过唾液酸产生药理活性。
OBJECTIVE: To compare the biological activity of sialic acid monomers and to reveal the relationship between sialic acid and mesalamine. Methods: The rabbit erythrocyte membrane calmodulin as a means. Results: NeuC (> 200μmol / L) and N-glucose neurotransmitter (NeuGc> 50μmol / L) significantly inhibited the activity of calmodulin (CaM) in rabbit erythrocyte membrane in vitro (P < 0.05). Inhibition rate and the concentration of the basic logarithmic linear. The inhibitory strength of NeuGc is about 2 ~ 4 times of 5AcNeu. In addition, 5AcNeu (200μmol / L) increased the inhibition of CaM at low concentrations (25-100μmol / L), since at this concentration, mapromazine failed to show anti-rabbit CaM activity. Conclusion: This work further proved that sialic acid monomer with anti-cellular calmodulin role. Mpromazine may produce pharmacological activity through sialic acid.