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目的研究氨基酚类化合物对人外周血淋巴细胞及小鼠脾脏细胞DNA的损伤效应。方法选用人淋巴细胞及小鼠脾脏细胞进行研究。暴露时间为1h。应用单细胞凝胶电泳技术,计算细胞损伤率及专用单位。结果3种氨基酚类化合物均能引起两种细胞不同程度的DNA损伤,高剂量组与阴性对照组相比,差异均有显著性(P<0.05),其中对氨基酚的毒性高于邻氨基酚和间氨基酚,其损伤的程度随剂量的增加而增加。结论与小鼠脾脏细胞相比,人外周血淋巴细胞的敏感性较强,更能直接反映氨基酚类化合物对人群的遗传毒性。
Objective To study the DNA damage effects of aminophenols on human peripheral blood lymphocytes and mouse spleen cells. Methods Human lymphocytes and mouse spleen cells were selected for study. Exposure time 1h. Single cell gel electrophoresis technique was used to calculate cell damage rate and specific unit. Results All the three aminophenols could induce DNA damage at different degrees in both cells. There was significant difference between the high-dose group and the negative control group (P <0.05), and the toxicity of aminophenol was higher than that of the adjacent amino group Phenol and m-aminophenol, the degree of injury increased with increasing dose. Conclusion Compared with mouse spleen cells, human peripheral blood lymphocytes are more sensitive and can directly reflect the genotoxicity of aminophenols to the human population.