论文部分内容阅读
目的建立三黄泻心汤中黄芩苷含量的高效毛细管电泳内标测定方法,比较3种三黄泻心汤不同制备方式样品中黄芩苷含量差异。方法以3,5-二硝基苯甲酸为内标物,熔融石英毛细管柱为分离通道(75μm×53.5 cm,有效长度为45.3 cm);40 mmol·L-1的硼砂(pH=3.0)作为缓冲溶液;DAD检测器,检测波长280 nm;工作电压为15 kV;压力进样:5 kPa,15 s,电解质封口:1 kPa,5 s;柱温15℃;每次进样前用缓冲液冲柱10 min。结果当3,5-二硝基苯甲酸内标溶液的浓度为0.061 0 mg·ml-1时,黄芩苷在4.05~74.89μg·ml-1范围内线性关系良好;通过测定,配方浸膏中黄芩苷含量明显高于传统汤剂和复方浸膏中的含量。结论该方法准确可靠,重复性好,适合复方制剂中黄芩苷的含量测定。从化学成分角度分析,配方浸膏中黄芩苷含量较传统汤剂和复方浸膏存在明显差异,可为临床应用提供科学依据。
Objective To establish a method for the determination of baicalin in Sanhuangxiexin Decoction by high performance capillary electrophoresis, and to compare the content of baicalin in three different preparations of Sanhuang Xiexin Decoction. Methods Using 3,5-dinitrobenzoic acid as internal standard, fused silica capillary column was used as separation channel (75 μm × 53.5 cm, effective length 45.3 cm) and 40 mmol·L -1 borax (pH = 3.0) Buffer solution; DAD detector, detection wavelength 280 nm; working voltage of 15 kV; pressure injection: 5 kPa, 15 s, electrolyte seal: 1 kPa, 5 s; column temperature 15 ℃; Punching column 10 min. Results When the internal standard solution of 3,5-dinitrobenzoic acid was 0.061 0 mg · ml-1, the linearity of baicalin was good in the range of 4.05-74.89 μg · ml-1. Through the determination, Baicalin content was significantly higher than the traditional decoction and compound extract content. Conclusion The method is accurate and reproducible, and is suitable for the determination of baicalin in compound preparations. From the chemical composition point of view, the contents of baicalin extracts than traditional decoction and compound extract significant differences, which can provide scientific basis for clinical application.