目的　观察目的基因在玻璃体增生膜的表达情况 ,以探讨基因治疗增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)的可行性。　方法　用 β-半乳糖苷酶基因作为报告基因 ,以逆转录病毒载体携带 ,直接注入 PVR模型眼玻璃体腔中 ,观察其在 PVR眼各组织表达情况。　结果　基因转染后可见玻璃体增生膜组织有转染基因表达 ,表达主要位于增生膜的表面 ,而视网膜组织及其它眼组织未见表达。　结论　逆转录病毒载体用于 PVR的基因治疗有靶向性作用 ,表明 PVR基因治疗具有可行性 Objective To observe the expression of the target gene in the proliferative vitreoretinopathy and to explore the feasibility of gene therapy for proliferative vitreoretinopathy (PVR). Methods The β-galactosidase gene was used as a reporter gene and was carried by retroviral vector and injected directly into vitreous cavity of PVR model eye to observe its expression in various tissues of PVR eye. Results After transfection, the gene expression of transfection was observed in the proliferative membrane of the vitreous. The expression was mainly located on the surface of the proliferative membrane, but not in the retina and other ocular tissues. Conclusion The retroviral vector has a targeted role in the gene therapy of PVR, indicating that PVR gene therapy is feasible