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以小麦-黑麦1BL·1RS易位系(Kavkaz、山农030-1)、1AL·1RS易位系(Amigo)、荆州黑麦、八倍体小黑麦劲松49、1R-7R二体异附加系以及普通小麦中国春、辉县红、铭贤169、Chancellor等为材料,对65个黑麦1RS特异标记进行鉴定,从中筛选出8个稳定的标记,即NOR-1、SECA2/SECA3、SCSS30.2、Sec1Gene、Sec1Pro、ω-Sec-P1/P2、ω-Sec-P3/P4和IB-267,可用于检测1AL·1RS易位系或1BL·1RS易位系;另外3个特异标记O-SEC5′-A/O-SEC3′-R、IAG95-1和SCM-9可用于区别1RS来源不同的1AL·1RS和1BL·1RS易位系。利用这11个标记和染色体原位杂交技术对40份山东省近年育成小麦品种(系)进行检测,发现潍麦8号、鲁麦14、济宁13、山农664、山农优麦3号和烟农25为1BL·1RS易位系,而且是1RS的整臂易位系,未检测到1AL·1RS易位系和其他易位类型。
The wheat rye 1BL · 1RS translocation lines (Kavkaz, Shannon 030-1), 1AL · 1RS translocation lines (Amigo), Jingzhou rye, octagonal triticale Jinsong 49, 1R-7R Additional lines and common wheat Chinese Spring, Huixian Hong, Mingxian 169, Chancellor and other materials for the identification of 65 rye 1RS specific markers, screening out of which eight stable markers, namely NOR-1, SECA2 / SECA3, SCSS30.2, Sec1Gene, Sec1Pro, ω-Sec-P1 / P2, ω-Sec-P3 / P4 and IB-267 can be used to detect the 1AL · 1RS or 1BL · 1RS translocations; the other 3 specific markers O-SEC5’-A / O-SEC3’-R, IAG95-1 and SCM-9 can be used to distinguish 1AL.1RS and 1BL.1RS translocation lines of different origin from 1RS. Forty wheat cultivars (lines) bred in recent years in Shandong Province were detected using the 11 markers and chromosomal in situ hybridization. Weimai 8, Lumai 14, Jining 13, Shannong 664, Shannongyou 3 and Yannong 25 is a 1BL · 1RS translocation line and is a whole-arm translocation line of 1RS, with no detection of the 1AL · 1RS translocation line and other translocation types.