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目的研究没食子酸(gallic acid,GA)体外诱导人肝癌SMMC-7721细胞凋亡及其作用机制。方法体外培养人肝癌SMMC-7721细胞,噻唑蓝(MTT)法观察细胞增殖;Hoechst-33258、AnnexinV-FITC/PI检测细胞凋亡情况;逆转录聚合酶链式反应(RT-PCR)法研究凋亡抑制基因Survivin mRNA的变化。结果 6.25~50μmol·L-1GA可抑制SMMC-7721细胞的生长,并呈剂量依赖性;不同剂量的GA作用48 h后,SMMC-7721细胞出现明显的凋亡形态,细胞凋亡率有显著的剂量依赖性。RT-PCR法检测结果显示,GA能明显抑制肝癌细胞中Survivin mRNA的表达。结论 GA可抑制人肝癌SMMC-7721细胞的增殖,这可能与下调凋亡抑制基因Survivin有关。
Objective To study the apoptosis of human hepatoma SMMC-7721 cells induced by gallic acid (GA) and its mechanism. Methods Human hepatocellular carcinoma SMMC-7721 cells were cultured in vitro. Cell proliferation was observed by MTT assay. Apoptosis was detected by Hoechst-33258 and Annexin V-FITC / PI. Reverse transcription polymerase chain reaction (RT-PCR) Changes of Survivin mRNA in Human Gastric Cancer Cells. Results 6.25 ~ 50μmol·L-1GA inhibited the growth of SMMC-7721 cells in a dose-dependent manner. After treated with different doses of GA for 48 h, the morphological changes of SMMC-7721 cells were observed. The apoptotic rate of SMMC-7721 cells was significant Dose-dependent. The results of RT-PCR showed that GA could significantly inhibit the expression of Survivin mRNA in hepatoma cells. Conclusion GA can inhibit the proliferation of human hepatocellular carcinoma SMMC-7721 cells, which may be related to the downregulation of the inhibitor of apoptosis Survivin.