目的 :提出一种自动测量大鼠脑梗死面积及体积的方法 ,克服人工勾画法和手动设定阈值法的不足。方法 :基于图像处理和分析系统的自编应用程序可根据脑片图像的灰度特征自动生成阈值 ,确定梗死灶。结果 :自动阈值法所得数据和标准值相关性良好 (γ =0 966,n =75 ) ;且多人多次重复测量所得数据的离散程度明显低于其他方法。结论 :自动阈值法可用于梗死区域的检测 ,且重复性好 OBJECTIVE: To propose a method for automatically measuring the area and volume of cerebral infarction in rats and to overcome the deficiencies of manual and manually set threshold methods. Methods: A self-developed application based on image processing and analysis system automatically generates thresholds based on the gray scale features of brain slice images to determine infarct size. Results: The data obtained by the automatic threshold method had good correlation with the standard values ​​(γ = 0 966, n = 75). The discreteness of multiple repeated measurements was significantly lower than that of other methods. Conclusion: The automatic threshold method can be used to detect the infarct area with good repeatability