本实验成功地建立了睾丸ＩＬ－１β原位杂交技术，并在此基础上探讨了输精管结扎对睾丸ＩＬ－１βｍＲＮＡ表达的影响。结果表明：输精管结扎６ｍｏ组家兔睾丸ＩＬ－１βｍＲＮＡ的杂交信号明显地强于假手术对照组，而结扎２５ｍｏ组恢复同龄假手术组水平。杂交信号多分布在曲细精管内的支持细胞和生殖细胞中，有的分布在间质的细胞中。结论：输精管结扎早期睾丸产生ＩＬ－１确有明显增加，２５ｍｏ恢复对照组水平。 The experiment successfully established testis IL-1β in situ hybridization technique, and on this basis to explore the effect of vasectomy on testicular IL-1βmRNA expression. The results showed that the hybridization signal of IL-1β mRNA in testis of 6-month vasectomy group was significantly stronger than sham-operated control group, while the 25-month ligation group recovered the same level of sham operation group. Hybridization signals are distributed in the seminiferous tubules supporting cells and germ cells, and some distributed in the stromal cells. Conclusion: IL-1 did not obviously increase in testes at early stage of vasectomy, and returned to control level at 25 months.