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用焦锑酸钾沉淀的电镜细胞化学方法研究低温胁迫下龙眼(DimocarpuslonganaLour.)幼叶细胞内Ca2+水平的变化。未经低温处理的幼叶,细胞壁、细胞间隙、质膜和液泡存在大量的Ca2+沉淀颗粒,叶绿体、细胞质和细胞核中也有一些Ca2+沉淀颗粒分布,表明液泡和细胞间隙是植物细胞的钙库。龙眼幼叶经4℃44h处理后,质膜上Ca2+沉淀颗粒明显增多,细胞质和细胞核中的Ca2+水平增加。不抗寒品种在低温胁迫下核膜开口,有时可观察到核内容物外漏。抗寒品种叶绿体中类囊体不形成基粒,少数片层结合重叠后伸展在整个叶绿体中。而不抗寒品种类囊体则堆积成明显的颗粒状基粒,片层数量较多,且叶绿体多数成蝶型排列。
The change of intracellular Ca2 + level in young leaves of Dimocarpus longana Lour. Under cold stress was studied by electron microscopy and cytochemical method using potassium pyroantimonate precipitation. There were a large number of Ca2 + precipitates in young leaves, cell wall, intercellular space, plasma membrane and vacuole without cryogenic treatment. Some Ca2 + precipitates also existed in chloroplast, cytoplasm and nucleus, indicating that the vacuole and intercellular space were calcium pools of plant cells. Longan leaf at 44 ℃ for 44h treatment, the plasma membrane Ca2 + precipitated particles increased significantly, the cytoplasm and nucleus of Ca2 + levels increased. Not cold-resistant varieties in the nuclear membrane opening under low temperature stress, and sometimes can be observed nuclear content leakage. The thylakids in cold-resistant chloroplasts do not form the basal grains, and the few laminae overlap and extend in the whole chloroplast. However, the non-resistant varieties of cysts are piled into a clear granular grana, lamellar number, and most of the chloroplasts into butterfly arrangement.