论文部分内容阅读
首先通过电聚合方法在玻碳电极表面制备了聚(3-甲基噻吩)(P3MT)修饰膜,然后在一定电位下将DNA分子电沉积到P3MT表面,制备了DNA/(P3MT)复合膜修饰玻碳电极.研究了8-羟基-2’-脱氧鸟嘌呤核苷(8-OH-dG)在该复合膜修饰电极上的伏安行为以及扫描速度、pH值和尿酸对其伏安行为和检测的影响.实验结果表明,该复合膜修饰电极结合了P3MT和DNA两者的优点,使8-OH-dG在复合膜修饰电极上的电化学行为明显改善,而且具有很好的重现性和稳定性.在0.1mol/LpH7.0的磷酸盐缓冲溶液(PBS)中,8-OH-dG的氧化峰电流与其浓度在0.28～4.2μmol/L和4.2～19.6μmol/L两个范围内成良好的线性关系,检出限为56nmol/L(S/N=3).该研究可以为制备HPLC或毛细管电泳电化学检测器检测8-OH-dG打下一定的基础,因此在检测尿样中8-OH-dG的研究方面具有潜在的应用价值.
Firstly, poly (3-methylthiophene) (P3MT) modified films were prepared on the surface of glassy carbon electrode by electropolymerization. Then DNA molecules were electrodeposited onto the surface of P3MT under a certain potential. DNA / (P3MT) Glassy carbon electrode.The voltammetric behavior of 8-hydroxy-2’-deoxyguanosine (8-OH-dG) on the composite film modified electrode and the effect of scanning speed, pH and uric acid on the voltammetric behavior The experimental results show that the composite membrane modified electrode combines the advantages of both P3MT and DNA, and the electrochemical behavior of 8-OH-dG on the composite membrane modified electrode is obviously improved, and has good reproducibility And stability in the phosphate buffer solution (PBS) of 0.1mol / LpH7.0, the oxidation peak current of 8-OH-dG and its concentration in the range of 0.28 ~ 4.2μmol / L and 4.2 ~ 19.6μmol / L two ranges The detection limit was 56 nmol / L (S / N = 3) .This study could provide a basis for the determination of 8-OH-dG by preparative HPLC or capillary electrophoresis electrochemical detector. Therefore, In 8-OH-dG research has potential applications.