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背景:对氧磷酶1(PON1)是一种抗氧化酶,能防止高密度脂蛋白(HDL)与低密度脂蛋白(LDL)氧化。关于运动对PON1活性的作用及其与PON1表型关系的研究还十分有限。本研究调查中年女性PON1-192表型、PON1活性、有氧运动、血脂水平、脂蛋白浓度间的关系。方法:从300名高加索人种女性中选取91名受试者,分为2组:平时进行规律有氧运动的运动组(n=50)、对照组(n=41)。用标准酶催化法确定血清PON1、盐刺激PON1(SSPON1)、芳香肌酯酶(ARE)活性,胆固醇水平,总HDL与HDL亚类的胆固醇水平与ARE活性(血清混合聚乙二醇分离出上清液),以及血脂水平、脂蛋白浓度用血清SSPON1(ARE)活性比率确定PON1-192QQ(低活性)、QR(中活性)、RR(高活性)表型组别。QR与RR联合组成RC表型组。结果:运动组血脂水平和脂蛋白浓度均高于对照组。如果不考虑表型,运动组与对照组血清PON1、SSPON1、HDL亚类相关的ARE活性均无显著差异(p>0.05)。然而,运动组中QQ表型女性PON1活性显著高于对照组(p<0.01),而RC表现组与对照组无差异。PON1表型(QQ组与RC组)与锻炼情况(运动组与对照组)对PON1活性的作用间存在显著交互作用。结论:规律性的有氧运动方案可根据PON1-192表型、而非血脂水平或浓度提升土耳其中年女性的PON1活性。
Background: Paraoxonase 1 (PON1) is an antioxidant enzyme that prevents the oxidation of high-density lipoproteins (HDL) and low-density lipoproteins (LDL). The effect of exercise on the activity of PON1 and its relationship with the phenotype of PON1 is still very limited. This study investigated the relationship between PON1-192 phenotype, PON1 activity, aerobic exercise, blood lipid levels, and lipoprotein concentrations in middle-aged women. METHODS: Totally 91 subjects from 300 Caucasians were divided into 2 groups: exercise group (n = 50) exercising regular aerobic exercise and control group (n = 41). Serum PON1, salt-stimulated PON1 (SSPON1), myo-esterase (ARE) activity, cholesterol levels, total and HDL-HDL subclinical cholesterol levels and ARE activity were determined by standard enzyme catalysis (serum mixed polyethylene glycol was isolated Serum), and lipid levels, lipoprotein concentrations PON1-192QQ (low activity), QR (medium activity), RR (high activity) phenotype groups were determined using the serum SSPON1 (ARE) activity ratio. QR and RR combined to form the RC phenotype group. Results: The levels of lipid and lipoprotein in exercise group were higher than those in control group. Without considering the phenotype, there was no significant difference (P> 0.05) in the activity of ARE related to the serum PON1, SSPON1 and HDL subclasses in exercise group and control group. However, the PON1 activity of QQ phenotype women in exercise group was significantly higher than that in control group (p <0.01), while there was no difference between RC appearance group and control group. There was a significant interaction between PON1 phenotype (QQ group and RC group) and exercise status (exercise group vs control group) on PON1 activity. CONCLUSION: Regular aerobic exercise regimen increases PON1 activity in middle-aged Turkish women based on PON1-192 phenotype, but not on the level or concentration of blood lipids.