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对热不稳定的极性有机化合物的结构和分子量测定,一直是有机质谱学面临的困难课题。过去,场解吸技术是获得这类化合物分子量的主要方法。但是,它要求复杂的设备和较高的操作技巧,且不能给出有结构特征的碎片离子。McLafferty等首先报道了难挥发分子的解吸化学电离(Desorption chemical ionization,DCI)。后有人用聚四氟乙烯惰性表面和活化过的场解吸丝作载体,研究了高至五肽的各种多肽及苯甲酸钠盐和钾盐。Rapp等用铼丝成功地获得了薯蓣皂苷(M_r868)及三肽(BacProLeuGlyOMe,M_r399)等的DCI谱。
The structure and molecular weight determination of thermally unstable polar organic compounds have always been a difficult issue for organic mass spectrometry. In the past, field desorption was the main method to obtain the molecular weight of these compounds. However, it requires complex equipment and high operating skills, and can not give structural ions to the fragment. McLafferty et al. First reported Desorption chemical ionization (DCI) of difficult-to-volatile molecules. After using PTFE inert surface and activated field desorption silk as a carrier, studied a variety of peptides up to pentapeptide and benzoic acid sodium salt and potassium salt. Rapp et al. Successfully obtained DCI spectra of diosgenin (M_r868) and tripeptides (BacProLeuGlyOMe, M_r399) using rhenium wire.