干扰素在抗病毒及抗肿瘤免疫中的作用日益受到重视。有关干扰素的测定方法很多,常用的有细胞病变抑制法、空斑减数法、染料吸收法。近来有人报道用同位素标记尿嘧啶核苷掺入法微量测定干扰素。本文报道用微量细胞培养法(下称微量法)测定干扰素,并与试管法作比较,探讨微量法的主要影响因素及其敏感性与可重复性。材料和方法一、微量细胞培养板:用4×10孔聚苯乙烯板(上海塑料三厂),孔径为7毫米,孔深 The role of interferon in antiviral and anti-tumor immunity is gaining more and more attention. Interferon determination of many ways, commonly used cytopathic effect inhibition method, plaque reduction method, dye absorption method. Recently, it has been reported that isotope labeled uridine incorporation of trace determination of interferon. This article reports the determination of interferon by micro-cell culture (hereinafter referred to as micro-method), and compared with the test tube method to explore the main influencing factors of trace method and its sensitivity and repeatability. Materials and methods First, the trace cell culture plate: with 4 × 10 holes polystyrene board (Shanghai Plastic Factory), a pore diameter of 7 mm, hole depth