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为了提高两相厌氧发酵工艺的生产性能,该文研究了一种新的两相半干法厌氧发酵沼气生产系统,即在水解相中批式发酵,每次从工作容积为4L的产烷相中取500mL接种液,与食品废物混合后发酵1～2d,再全部输入产烷相继续生产沼气,如此循环。实验运行温度为50℃,食品废物总固体TS和挥发性固体VS分别为19.3%和16.1%,产烷相先在4gVS·d-1的进料率下单相运行至稳定,稳定期日产气量为(4.09±0.03)L(甲烷占69.0%),pH为7.50±0.02,TS和VS分别为(1.05±0.02)%和(0.64±0.02)%。稳定的产烷相与水解相联合后,水解相在60～700g的进料量下都能正常产气,但氢气含量较低。产烷相在进料量600g时产气量最高,为29.1L/d,随后受到抑制,受抑制前的甲烷含量为72.4%～74.0%,TS和VS含量也在该进料量后达到最高峰,分别为3.50%和2.32%。研究结果表明新的两相半干法厌氧发酵沼气生产系统有效提高了生产性能,在今后的研究中,需要降低从产烷相输入水解相的接种液量,以更好抑制水解相产烷微生物,提高产氢性能,还可使产烷相更加稳定,承受更高的进料量。
In order to improve the production performance of two-phase anaerobic fermentation process, a new bi-phase anaerobic fermentation biogas production system was studied in this paper, that is batch fermentation in the hydrolysis phase, each time from the working volume of 4L Take 500mL alkane phase inoculum, and food waste mixed fermentation 1 ~ 2d, and then all the input of methane continue to produce biogas, so cycle. The experimental operating temperature was 50 ℃, the total TS and volatile solids VS of the food wastes were 19.3% and 16.1%, respectively. The alkane phase was first run from single phase to stable at the feed rate of 4gVS · d-1, and the steady- (4.09 ± 0.03) L (methane accounted for 69.0%), pH was 7.50 ± 0.02, TS and VS were (1.05 ± 0.02)% and (0.64 ± 0.02)%, respectively. When the stable alkane phase is combined with hydrolysis, the hydrolyzed phase can produce gas normally with the feed amount of 60 ~ 700g, but the hydrogen content is low. The gas yield of the alkane phase was the highest at 29Og / g at feed rate of 600g, and then was inhibited, and the methane content before inhibition was 72.4% ~ 74.0%. The TS and VS contents reached their peak after the feed rate , 3.50% and 2.32% respectively. The results show that the new two-phase semi-dry anaerobic fermentation biogas production system effectively improves the production performance. In the future research, it is necessary to reduce the amount of inoculation liquid input into the hydrolysis phase from the alkane phase to better inhibit the production of hydrocarbons Microorganisms, improve hydrogen production performance, but also to produce more stable phase of alkane, to withstand higher feeding amount.