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采用药物与DNA作用的体外检测方法，以DNA嵌入剂表阿霉素（EDR）作对照，从分子药理水平研究雷公藤内酯醇（TL）对乳腺癌细胞DNA的作用模式。实验发现TL在120μg／ml浓度，37℃作用24h可与DNA模板结合，同EDR呈嵌合效应，但嵌入DNA尚不牢固，易发生解离；EDR在较低浓度（50μg／ml）作用30min即可显示明显的嵌合效应且是不可逆的。说明TL对乳腺癌细胞DNA的作用模式为嵌合逆行模型，嵌合能力远低于EDR，与DNA结合亦不可能是其抗癌主效应。
Using the in vitro detection method of drug and DNA and the DNA intercalator epirubicin (EDR) as a control, the effect of triptolide (TL) on the DNA of breast cancer cells was studied from the molecular level of pharmacology. It was found that TL could bind to the DNA template at the concentration of 120 μg/ml at 37°C for 24 h, showing a chimeric effect with EDR, but the inserted DNA was not yet stable and dissociated easily; EDR at a lower concentration (50 μg/ml) for 30 min. Can show a clear chimera effect and is irreversible. This shows that the effect of TL on the DNA of breast cancer cells is a chimeric retrograde model. The chimerism is much lower than that of EDR, and binding to DNA is not the main anticancer effect.