测定人工北虫草及废料酶解液中腺苷和虫草素含量,判断酶解过程对腺苷和虫草素的影响。以kromasil 5u C18色谱柱,甲醇-水为流动相,采用梯度洗脱,流速0.8 mL/min,检测波长260 nm,柱温30℃。人工北虫草废料酶解液中腺苷和腺苷的含量为11.24 mg/L和70.40 mg/L。废料酶解液中含有一定量的腺苷和虫草素,具有利用价值。 The content of adenosine and cordycepin in artificial Cordyceps militaris and waste hydrolyzate was determined to determine the effect of enzymolysis on adenosine and cordycepin. The gradient elution was performed on a kromasil 5u C18 column with methanol-water as the mobile phase at a flow rate of 0.8 mL / min. The detection wavelength was 260 nm and the column temperature was 30 ℃. The contents of adenosine and adenosine in the enzymatic hydrolyzate of Artificial Cordyceps militaris were 11.24 mg / L and 70.40 mg / L, respectively. Waste hydrolyzate contains a certain amount of adenosine and cordycepin, has the use value.