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目的探讨子痫前期患者血清瘦素、C-反应蛋白(C-reactive protein,CRP)、干扰素诱导蛋白10(inducible protein10,IP-10)、特异性受体可溶性络氨酸激酶受体1(soluble fms-like tyrosine kinase-1,sFlt-1)/血清胎盘生长因子(placental growth factor,PLGF)的关系。方法用ELISA检测3组血清中leptin水平法测定66例子痫前期孕妇、60例正常孕妇、58例健康非孕妇空腹血清瘦素水平及IP-10浓度,用酶联免疫吸附实验双抗体夹心法,分别检测血清PLGF、sFlt-1水平;血清CRP浓度的测定采用TBA-40FR型全自动生化分析仪免疫比浊法进行测定。结果子痫前期患者血清瘦素浓度显著高于健康孕妇;健康孕妇血清中瘦素水平与血清CRP浓度、血清IP-10浓度有正相关关系(相关系数r1、r2分别为0.34、0.40),在子痫前期患者,血清瘦素水平和IP-10呈正相关关系(r=0.64);瘦素水平升高及sFlt-1/PlGF比值升高与病情严重程度呈正相关关系(r=0.70、0.72)。结论子痫前期血清瘦素水平高于正常孕妇,无论正常孕妇还是子痫前期孕妇其血清瘦素浓度升高与IP-10密切相关,提示:血清瘦素升高通过IP-10的抗血管生成作用导致了血管内皮功能障碍,而且,瘦素浓度升高及sFlt-1/PlGF比值升高增加了子痫前期发生的危险,与病情严重程度呈正相关,有可能成为监测子痫前期病情变化及预后的指标。
Objective To investigate the expression of serum leptin, C-reactive protein (CRP), inducible protein10 (IP-10), and specific receptor soluble tyrosine kinase receptor 1 ( soluble fms-like tyrosine kinase-1, sFlt-1) / serum placental growth factor (PLGF). Methods The levels of leptin and IP-10 in 66 pregnant women, 60 normal pregnant women and 58 healthy pregnant women were measured by ELISA. The levels of leptin and IP-10 in serum were detected by enzyme-linked immunosorbent assay (ELISA) Serum levels of PLGF and sFlt-1 were detected respectively. The serum CRP level was determined by TBA-40FR automatic biochemical analyzer. Results Serum leptin levels in patients with preeclampsia were significantly higher than those in healthy pregnant women. Serum levels of leptin in healthy pregnant women were positively correlated with serum levels of CRP and serum IP-10 (r1, r2 = 0.34, 0.40, respectively) There was a positive correlation between serum leptin level and IP-10 in preeclampsia patients (r = 0.64). Elevated leptin level and sFlt-1 / P1GF ratio were positively correlated with severity of disease (r = 0.70,0.72) . Conclusions Serum leptin level in preeclampsia is higher than that in normal pregnant women. Serum leptin level is closely related to IP-10 in normal pregnant women and pregnant women with preeclampsia, suggesting that elevated serum leptin may be related to anti-angiogenesis via IP-10 The role of vascular endothelial dysfunction, and, leptin concentration and increased sFlt-1 / PGF ratio increased the risk of preeclampsia, and the severity of the disease was positively correlated, it may become monitoring changes in preeclampsia and Prognosis index.