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【目的】探讨榴莲皮提取物(DPE)的抗炎效果及其作用机制。【方法】采用角叉菜胶致小鼠足跖肿胀及2,4-二硝基氟苯(2,4-DNFB)引起小鼠变应性接触性皮炎实验模型评价DPE的体内抗炎效果;采用四甲基偶氮唑盐(MTT)法以及脂多糖(LPS)诱导的RAW 264.7细胞炎症模型评估体外抗炎效果。【结果】在体内实验,与空白组比较,DPE各剂量组对角叉菜胶诱发的小鼠足跖肿胀抑制效果显著(P<0.01或P<0.001),DPE对2,4-DNFB所致小鼠变应性接触性皮炎也呈现明显的抑制作用,且作用效果具有较好的量效关系。在体外实验,在给定的浓度下DPE不影响细胞增殖;25 mg/L与50 mg/L DPE可有效地抑制炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、一氧化氮(NO)以及转录因子核因子-кВ(NF-кВ)含量,提高白细胞介素-10(IL-10)等抗炎因子含量。【结论】DPE具有较好的抗炎作用,其作用可能与DPE抑制NF-кВ信号通路相关。
【Objective】 To investigate the anti-inflammatory effect and mechanism of durian bark extract (DPE). 【Method】 The in vivo anti-inflammatory effects of DPE were evaluated by carrageenin-induced paw edema in mice and experimental mice model of allergic contact dermatitis caused by 2,4-dinitrofluorobenzene (2,4-DNFB) In vitro anti-inflammatory effects were evaluated using a MTT assay and lipopolysaccharide (LPS) induced RAW 264.7 cell inflammation model. 【Result】 In vivo, compared with the blank group, each dose of DPE had a significant inhibitory effect on carrageenan-induced paw edema in mice (P <0.01 or P <0.001). DPE induced 2,4-DNFB Allergic contact dermatitis in mice also showed significant inhibition, and the effect has a good dose-effect relationship. In vitro, DPE did not affect cell proliferation at a given concentration. 25 mg / L and 50 mg / L DPE could effectively inhibit the inflammatory cytokines TNF-α, IL- (IL-1β), nitric oxide (NO), and nuclear factor-kappa (NF-κB), and to increase anti-inflammatory cytokines such as interleukin-10 content. 【Conclusion】 DPE has a good anti-inflammatory effect, which may be related to DPE inhibiting NF-кВ signaling pathway.