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目的：探讨小儿急性淋巴细胞白血病（ＡＬＬ）免疫球蛋白重链（ＩｇＨ）基因重排形式的种类，以及是否存在几个克隆并存的情况。方法：应用多聚酶链反应（ＰＣＲ）和单链构象多态性（ＳＳＣＰ）技术，构建ＩｇＨ－ＰＣＲ－ＳＳＣＰ基因指纹（ｆｉｎｇｅｒｐｒｉｎｔｉｎｇ），分析３４例ＡＬＬ患儿ＩｇＨ基因重排的基因指纹图谱。结果：单克隆的ＩｇＨ基因重排中除了单等位基因重排９例（３２．１４％）外，有１５例（５３．５７％）是双等位基因重排；４例（１４．２９％）白细胞数高、免疫表型是早期Ｂ细胞的病例表现寡克隆性ＩｇＨ重排。结论：从基因水平证实，小儿ＡＬＬ多发生ＩｇＨ双等位基因重排，且有多个克隆并存的现象。
Objective: To investigate the types of immunoglobulin heavy chain (IgH) gene rearrangements in pediatric acute lymphoblastic leukemia (ALL) and the existence of several colonies coexisting. Methods: IgH-PCR-SSCP fingerprinting was constructed by polymerase chain reaction (PCR) and single-strand conformation polymorphism (SSCP). The gene fingerprinting of IgH gene rearrangements in 34 children with ALL was analyzed. RESULTS: Fifteen patients (53.57%) had dual allele rearrangements in addition to 9 (32.14%) single-gene rearrangements in IgH gene rearrangements; 4 (14.29% %) High number of white blood cells, immunophenotype is the case of early B cell oligoclonal IgH rearrangement. Conclusion: It is confirmed from the gene level that IgH dual allele rearrangement occurs in children with ALL, and multiple clones co-exist.