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目的研究全基因组ＤＮＡ稀有位点限制性内切酶酶切脉冲电场电泳图谱（ＧＭ－ＰＦＧＥ）在铜绿假单胞菌基因分型中的应用，并与表型分型比较。方法对１个月内来自两个医院的病人及环境的２０株铜绿假单胞菌进行了ＧＭ－ＰＦＧＥ图谱分析，同时进行３０种生化反应的统计分型、２３种药物的敏感性分型、胞外脂多糖的血清抗原分型；并利用数值分类软件包进行相关性比较研究。结果临床致病铜绿假单胞菌的生化表型基本稳定，但其药物抗性的获得与丢失较为明显。血清型、生化性状及药物抗性之间无明显对应关系。当２菌株ＧＭ－ＰＦＧＥ图谱条带相似系数大于８０％时，为同一菌株的不同克隆亚型，当相似系数在２５％～７０％之间时，则为不同菌株。结论ＧＭ－ＰＦＧＥ分析可显示染色体结构的区域多型性，其重复性好，分辨率明显高于表型分型，结果可靠，必将成为铜绿假单胞菌或其它病原微生物分子流行病学研究的有力工具。
Objective To study the genotyping of Pseudomonas aeruginosa with restriction endonuclease digestion and whole-genome DNA restriction mapping (GM-PFGE) and to compare with the phenotype. Methods The GM-PFGE patterns of 20 strains of Pseudomonas aeruginosa from two hospitals in two hospitals within one month were analyzed. Meanwhile, the statistical classification of 30 biochemical reactions, the sensitivity typing of 23 drugs, Extracellular lipopolysaccharide serum antigen typing; and the use of numerical classification software packages for comparative study. Results The biochemical phenotype of clinically pathogenic Pseudomonas aeruginosa was basically stable, but the gain and loss of drug resistance were more obvious. Serum type, biochemical traits and drug resistance no significant correspondence between. When the similar coefficient of band of GM-PFGE of 2 strains was more than 80%, they were different clonal subtypes of the same strain. When the similarity coefficient was between 25% and 70%, different strains were found. Conclusion The results of GM-PFGE analysis showed that the polymorphism of chromosome structure was good, its repeatability was good, the resolution was obviously higher than that of phenotyping, and the results were reliable and would be the molecular epidemiological study of Pseudomonas aeruginosa or other pathogenic microorganisms A powerful tool.