论文部分内容阅读
目的:观察补肾中药复方首参颗粒对动脉粥样硬化模型大鼠血管病理及氧化应激的影响。方法:将36只SD大鼠随机分为正常组、模型组、中药组和西药组,每组9只。除正常组外,其余各组通过一次性腹腔注射维生素D3及连续饲喂高脂饮食的方法建立动脉粥样硬化大鼠模型。在造模的同时,中药组和西药组分别予灌胃首参颗粒、普伐他汀,共干预12周。实验结束,HE染色观察大鼠腹主动脉病理变化;酶联免疫吸附法(ELISA)检测大鼠血清髓过氧化物酶(MPO)、氧化低密度脂蛋白(oxLDL)、超敏C反应蛋白(hs-CRP)的变化。结果:病理检测显示,模型组有明显的动脉粥样硬化病变,而药物干预组血管病变明显减轻或消失;模型组MPO、oxLDL、hs-CRP含量较正常组显著升高(P<0.05或P<0.01),药物干预组MPO、oxLDL、hs-CRP含量较模型组显著降低(P<0.01),且中药组和西药组间无明显差异。结论:首参颗粒对动脉粥样硬化病变有明显的防治作用,其机制可能与调节氧化与炎症相关指标有关。
Objective: To observe the effect of Bushen Chinese medicine compound ginseng granules on the vascular pathology and oxidative stress in atherosclerotic model rats. Methods: 36 SD rats were randomly divided into normal group, model group, TCM group and western medicine group, with 9 rats in each group. In addition to the normal group, the rest of the group through a one-time intraperitoneal injection of vitamin D3 and continuous feeding of high-fat diet method to establish atherosclerosis rat model. At the same time of modeling, the traditional Chinese medicine group and the western medicine group were respectively given gavage of first stomach granules and pravastatin for a total of 12 weeks. At the end of the experiment, the pathological changes of the abdominal aorta were observed by HE staining. The levels of serum MPO, oxLDL and Hs-CRP in the rats were measured by enzyme-linked immunosorbent assay (ELISA) hs-CRP) changes. Results: Pathological examination showed that the model group had obvious atherosclerotic lesion, and the vascular lesions in the intervention group were significantly reduced or disappeared. The contents of MPO, oxLDL and hs-CRP in the model group were significantly higher than those in the normal group (P <0.05 or P <0.01). The levels of MPO, oxLDL and hs-CRP in the drug-treated group were significantly lower than those in the model group (P <0.01). There was no significant difference between the traditional Chinese medicine group and the western medicine group. Conclusion: The first ginseng granules have a significant preventive and therapeutic effect on atherosclerotic lesions. The mechanism may be related to the regulation of the indexes related to oxidation and inflammation.