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为探讨硫化氢(H2S)作为潜在的新型氮肥增效剂的可能性,通过水培实验,以扬麦16为材料,以NaHS为H2S供体(浓度0.01mmol·L-1),研究了外源低浓度H2S对低氮(2.5mmol·L-1)、中氮(7.5)和高氮(15mmol·L-1)条件下小麦幼苗光合作用、硝态氮吸收和同化的影响。结果表明,外源低浓度H2S促进了小麦幼苗的光合作用及对硝态氮的吸收,使低氮条件下植株干物质重增加15.5%,显著提高叶片可溶性蛋白、总氮及叶绿素含量。外源H2S处理后,低氮和中氮水平下叶片硝酸还原酶(NR)、谷氨酰胺合成酶(GS)以及谷氨酸脱氢酶(GDH)活性有所增加,尤其是在低氮水平下增加显著,但在高氮水平下NR与GS活性分别下降22%和13%,GDH活性与对照无明显差异。以上结果说明外源低浓度H2S可提高小麦幼苗对低氮的适应性,促进其生长及对氮素吸收与同化。
In order to explore the possibility of hydrogen sulfide (H2S) as a potential new nitrogen fertilizer synergist, the effects of hydrogen sulfide (H2S) as a potential new nitrogen fertilizer synergist were studied by hydroponics experiment with Yangmai 16 as the material and NaHS as the H2S donor (concentration 0.01mmol·L-1) Effects of low concentration H2S on photosynthesis, nitrate uptake and assimilation of wheat seedlings under low nitrogen (2.5 mmol·L-1), medium nitrogen (7.5) and high nitrogen (15 mmol·L-1) conditions. The results showed that exogenous low concentration of H2S promoted the photosynthesis and nitrate uptake of wheat seedlings, increased the dry weight of plant under low nitrogen condition by 15.5%, and significantly increased the contents of soluble protein, total nitrogen and chlorophyll in leaves. After exogenous H2S treatment, the activities of nitrate reductase (NR), glutamine synthetase (GS) and glutamate dehydrogenase (GDH) were increased under low and medium nitrogen levels, especially at low nitrogen level , While the activities of NR and GS decreased by 22% and 13% at high nitrogen level, respectively. There was no significant difference in the activity of GDH between the control and the control. The above results show that exogenous low concentration of H2S can improve the adaptability of wheat seedlings to low nitrogen and promote their growth and nitrogen uptake and assimilation.