论文部分内容阅读
目的 研究和比较不同肺癌细胞迁移、粘附等生物学行为 ,探讨肿瘤细胞的转移机制。方法 将 4株肺癌细胞培养在含 1 0 %热灭活小牛血清、青霉素 (1 0 0U/ml)和链霉素 (1 0 0 μg/ml)、 37℃、 5 %CO2 下的RPMI1 640培液中 ,然后按DeanSheppard方法测定细胞的粘附能力 ;用划痕法和琼脂滴法测定细胞迁移能力 ;同时测定各肺癌细胞的铺展、软琼脂集落形成能力、梯形DNA和细胞生长曲线。结果 95C的迁移能力、细胞铺展率和增殖率最高 ,95D、A4次之 ,H4 6 0 最弱 ;95D的粘附能力最强 ,其次为 95C、A4 ;H4 6 0 的粘附率最低。只有H4 6 0 出现较强的梯形DNA ;各株细胞软琼脂集落形成能力无显著差异。结论 不同肺癌细胞迁移和粘附能力存在差异。提示肺癌细胞的转移能力可能与其生物学行为有关。
Objective To study and compare the biological behaviors of different lung cancer cells such as migration and adhesion, and explore the mechanism of tumor cell metastasis. Methods Four lung cancer cells were cultured in RPMI1 640 containing 10% heat-inactivated calf serum, penicillin (100 U/ml) and streptomycin (100 μg/ml), 37°C, and 5% CO2. In the culture medium, the adhesion ability of the cells was measured by the Dean Sheppard method; the migration ability of the cells was determined by the scratch method and the agar drop method; and the spread of each lung cancer cell, the ability to form soft agar colonies, the trapezoidal DNA, and the cell growth curve were simultaneously measured. Results The migration ability, cell spreading rate and proliferation rate of 95C were the highest, followed by 95D, A4, and H4600 were the weakest; 95D had the strongest adhesion ability, followed by 95C, A4; H4600 had the lowest adhesion rate. Only H460 showed stronger trapezoidal DNA; there was no significant difference in the ability of soft agar colonies to form in each strain. Conclusion There are differences in the migration and adhesion of different lung cancer cells. It suggests that the metastatic potential of lung cancer cells may be related to its biological behavior.