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Calcitonin gene-related peptide (CGRP), a 37-amino acid neuropeptide, is produced in dorsal root ganglia (DRG) neurons, which extend nerves peripherally to different organs and centrally to the spinal cord.We have previously reported that plasma levels of CGRP are significantly elevated in rats after injection of lipopolysaccharide (LPS) in vivo.LPS triggers CGRP release from capsaicin-sensitive sensory nerves in the isolated mesenteric arteial bed of the rats.In this work, we used the cultured DRG neurons of neonatal mrs to detemine whether or not LPS could trigger CGRP release and if it did so, what cellular signaling pathway was involved in the CGRP release.Neurons were disassociated from the DRG of 5- 7-d neonatal rat using
Calcitonin gene-related peptide (CGRP), a 37-amino acid neuropeptide, is produced in dorsal root ganglia (DRG) neurons, which extend nerves peripherally to different organs and centrally to the spinal cord. We have previously reported that plasma levels of CGRP are significantly elevated in rats after injection of lipopolysaccharide (LPS) in vivo. LPS triggers CGRP release from capsaicin-sensitive sensory nerves in the isolated mesenteric arteial bed of the rats. In this work, we used the cultured DRG neurons of neonatal mrs to detemine whether or not LPS could trigger CGRP release and if it did so, what cellular signaling pathway was involved in the CGRP release. Neurons were disassociated from the DRG of 5- 7-d neonatal rat using