论文部分内容阅读
目的 :研究肝癌癌周组织淋巴细胞和肝癌TIL的分布及抗瘤活性。方法 :用冷胶原酶消化分离法来分离肝癌癌周组织淋巴细胞及肝癌实质部和中心部TIL并测定它们的获得率。用3H -TdR释放法测定它们对自体肝癌细胞的杀伤活性。结果 :肝癌实质部TIL获得率最高 ,肝癌癌周组织淋巴细胞获得率次之 ,肝癌中心部TIL获得率最低。它们对自体肝癌细胞的杀伤活性从高到低则分别为肝癌实质部TIL、肝癌中心部TIL和肝癌癌周组织淋巴细胞。结论 :肝癌癌周组织和肝癌组织不同部位的淋巴细胞分布有所不同。它们的抗瘤活性也各不相同。为了获得数量多和抗瘤活性高的淋巴细胞 ,应尽量从肝癌实质部来分离获取
Objective : To study the distribution and antitumor activity of TIL in peripheral lymphocytes and hepatocellular carcinoma of hepatocellular carcinoma. Methods : Cold collagenase digestion and isolation were used to isolate lymphocytes from hepatocellular carcinoma, the hepatic parenchyma and the central part of TIL, and their acquisition rate was determined. The 3H-TdR release assay was used to determine their killing activity against autologous hepatoma cells. Results: The highest rate of TIL was obtained in the substantial part of liver cancer, followed by the lymphocyte-acquisition rate of hepatocellular carcinoma and the lowest rate of TIL in the center of liver cancer. The killing activity of autologous hepatoma cells from high to low was the hepatic carcinoma TIL, hepatoma center TIL and hepatocarcinoma tissue lymphocytes. Conclusion : The distribution of lymphocytes in different tissues of hepatocellular carcinoma tissues and hepatic cancer tissues is different. Their anti-tumor activity also varies. In order to obtain a large number of lymphocytes with high anti-tumor activity, they should be isolated from the substantive part of liver cancer.