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一、概述应用激光全息照相显微技术,可以在极短的曝光时间内制得样品的全息照片.这种全息照片记录了样品的所有三维特点,样品不需要特殊制备,更不需要象用普通显微镜观察时那样要将样品制得很薄,以便使样品在观察时不在焦点内外漂浮.这种全息照相可以在很大景深范围内观察,而且观察到的再现象是立体的,并具有很高的放大倍数、高分辨力和高亮度,是一种详细记录生物和物理现象的有效方法.可以长期保存生物样品的三维结构和其详细细节的全息照片,作为完全没有变形的生物样本,便于以后随时对这种样品进行观察和分析之用.同时还可以记录生物体活动的情况.
I. INTRODUCTION The use of laser holographic microscopy to produce a holographic image of a sample in a very short exposure time, which records all the three-dimensional characteristics of a sample without the need for special preparation of the sample, To make the sample very thin during microscopic observation so that the sample does not float in and out of focus when viewed, this hologram can be viewed over a large depth of field and the observed re-appearance is stereoscopic and has high Magnification, high resolution and high brightness, is an effective method for detailed documentation of biological and physical phenomena.Height-time preservation of the three-dimensional structure of biological samples and the details of their holographic images as a completely undeformed biological specimen facilitates later This sample can be observed and analyzed at any time, and the activity of the organism can also be recorded.