运用免疫组化和原位杂交法对43例口腔鳞癌的 MTS1基因,即 p16的表达进行研究,并分析了口腔鳞癌细胞株 p16的表达。结果43例口腔癌中28例为 p16阳性,15例为 p16阴性,p16基因的改变是口腔鳞癌发展中的频发事件。组化和原位杂交结合能较好地反映肿瘤组织中 p16基因和蛋白的改变。研究表明 p16的改变可能是肿瘤发生的晚发事件,但和肿瘤的发生部位、浸润、转移、分级无关。TCA 细胞株是研究 P16表达的良好模型。 Immunohistochemistry and in situ hybridization of 43 cases of oral squamous cell carcinoma MTS1 gene, p16 expression, and analyzed the expression of oral squamous cell carcinoma cell line p16. Results Of the 43 oral carcinomas, 28 were positive for p16 and 15 were negative for p16. The alteration of p16 gene was a frequent event in the development of oral squamous cell carcinoma. Tissue and hybridization in situ hybridization can better reflect the tumor tissue p16 gene and protein changes. Studies have shown that changes in p16 may be late events of tumorigenesis, but has nothing to do with the tumor site, invasion, metastasis, grading. TCA cell line is a good model for studying P16 expression.