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用Li-6400光合仪同时测定了CO2浓度为380和600μmol·mol-1条件下黄山栾树的光响应曲线和快速光曲线,分别采用不同模型进行了拟合。结果表明:直角双曲线模型和双指数方程拟合得到的最大电子传递速率远大于实测值;直角双曲线模型、非直角双曲线模型、单指数方程不能拟合黄山栾树存在PSⅡ动力学下调的快速光曲线,只有双指数方程和直角双曲线修正模型可以拟合黄山栾树存在PSⅡ动力学下调的快速光曲线,且可以计算它的饱和光强。综合拟合结果可知,直角双曲线修正模型不仅可以很好地拟合黄山栾树的快速光曲线,而且得到的最大电子传递速率和饱和光强与实测值相符合。此外,通过拟合植物的快速光曲和光响应曲线,还可以判断在饱和光强时它的电子传递速率与碳同化是否同时达到最大值。
The light response curves and fast light curves of Huangshan Lonicerae were determined by Li-6400 photosynthesis system at different CO2 concentrations of 380 and 600 μmol · mol-1, respectively, and fitted with different models. The results show that the maximum electron transfer rate obtained by the fitting of the right-angle hyperbola model and the double-exponential equation is much larger than the measured values. The right-angle hyperbola model, the non-right-angle hyperbola model and the single exponential equation can not fit the dynamics of PSⅡ dynamics Light curve. Only the double exponential equation and the right hyperbola correction model can fit the rapid light curve with the PSⅡ kinetics down-regulated in the Huangshan Luan tree, and its saturated light intensity can be calculated. According to the fitting results, the rectangular hyperbola correction model can not only fit the fast light curve of Huangshan Luan tree well, but also get the maximum electron transfer rate and saturation light intensity in accordance with the measured values. In addition, by fitting the fast photoperiod and photoresponse curve of a plant, one can also determine whether its electron transfer rate and carbon assimilation reach a maximum at the same time at a saturated light intensity.