以高效液相色谱法作为羟基喜树碱含量检测方法,系统讨论了用超声法从喜树果实中提取羟基喜树碱用以制备分析样品的方法。采用HIQ sil C18柱(4.6×250 mm),流动相为乙腈:水(3:7,V:V),流速1 mL/min,检测波长266 nm。确定的最佳超声提取条件为:以60%乙醇为提取溶剂在60℃提取50 min。图3表1参10。 Using high performance liquid chromatography (HPLC) as a method for the determination of hydroxycamptothecin, we systematically discussed the method of extracting hydroxycamptothecin from Camptotheca acuminata by ultrasonic method to prepare analytical samples. A HIQ Sil C18 column (4.6 × 250 mm) was used. The mobile phase was acetonitrile: water (3: 7, V: V) at a flow rate of 1 mL / min and the detection wavelength was 266 nm. The optimal extraction conditions for ultrasonic extraction were as follows: extraction with 60% ethanol as extraction solvent at 60 ℃ for 50 min. Figure 3 Table 1 Reference 10.