论文部分内容阅读
为研究ns脉冲电场(nsPEF)治疗在体肿瘤的生物电效应机制,以接种人黑色素瘤细胞A375的BALB/c裸鼠为研究对象,采用电压幅值为4kV、脉冲宽度为200ns、重复频率为1Hz的脉冲电场进行处理。用凝胶电泳法检测脉冲处理后的DNA Ladder分布情况表明,与对照组相比,处理组有明显阶梯状分布,即细胞发生凋亡。用TUNEL法检测肿瘤组织的凋亡情况表明,处理组表现出较高凋亡率(检验水准P<0.01)。用Western blot法和免疫组织化学方法检测脉冲处理后肿瘤组织中促凋亡蛋白Bax、凋亡抑制蛋白Bcl-2的表达量情况,2者共同表明,与对照组相比,处理组Bax表达量显著升高(P<0.01),而Bcl-2表达量明显降低(P<0.01)。实验结果揭示nsPEF通过Bax、Bcl-2基因调控作用来诱导肿瘤凋亡。
In order to study the bioenergetic mechanism of nsPEF in vivo tumors, BALB / c nude mice inoculated with human melanoma A375 cells were used as research object. The voltage amplitude was 4kV, the pulse width was 200ns and the repetition frequency was 1Hz pulse electric field for processing. Detection of the distribution of DNA Ladder after the pulse treatment by gel electrophoresis showed that the treatment group had a marked ladder-like distribution compared with the control group, that is, the cell apoptosis occurred. TUNEL assay of tumor tissue apoptosis showed that the treatment group showed a higher rate of apoptosis (test level P <0.01). Western blot and immunohistochemistry were used to detect the expression of Bax and Bcl-2 in the tumor tissue after pulse treatment. Both of them showed that compared with the control group, the expression of Bax (P <0.01), while the expression of Bcl-2 was significantly decreased (P <0.01). The experimental results revealed that nsPEF induces tumor apoptosis through the regulation of Bax and Bcl-2 genes.