论文部分内容阅读
目的探讨汉族人群中存活素(survivin)基因rs8073069及rs1042489位点与原发性肝细胞癌(HCC)的相关性,为采取干预措施提供参考依据。方法采用病例对照研究方法对在江苏省海门市10个社区收集的176例汉族HCC病例和随机匹配的196名健康汉族人群进行一般情况问卷调查以及survivin基因rs8073069和rs1042489位点基因分型。结果 rs8073069位点CC、CG、GG基因型和C、G 2个等位基因在HCC病例组的频率分别为8.52%、35.22%、56.25%2、6.14%7、3.86%,在对照组的频率分别为6.12%3、9.80%、54.08%、26.02%7、3.98%,不同遗传模式下rs8073069位点基因型和等位基因分布在HCC病例组与对照组间分布差异均无统计学意义(P>0.05);rs1042489位点CC、CT、TT基因型和C、T 2个等位基因在HCC病例组的频率分别为17.61%、55.68%、26.70%、45.45%、54.55%,在对照组的频率频率为13.78%、52.04%、34.18%、39.80%、60.20%,不同遗传模式下rs1042489位点基因型及等位基因在HCC病例组与对照组间分布差异均无统计学意义(P>0.05);多因素Logistic回归分析结果表明,在相加遗传模式下,HBsAg+和有乙肝史是HCC的危险因素r,s8073069G-rs1042489T单倍型是HCC的保护因素;不同遗传模式下环境因素与单倍型交互作用分析结果表明,显性、隐性和相加遗传模式均未发现单倍型与环境因素的交互作用(P>0.05)。结论汉族人群survivin基因rs8073069和rs10424892位点多态性与HCC的易感性无关;rs8073069G-rs1042489T单倍型是HCC的保护单倍型。
Objective To investigate the association between the survivin rs8073069 and rs1042489 loci in Han population and primary hepatocellular carcinoma (HCC), and to provide a reference for the intervention. Methods A case-control study was conducted to investigate the general situation of 176 HCC cases and 196 healthy Han nationalities collected from 10 communities in Haimen, Jiangsu Province, and to genotype rs8073069 and rs1042489 of survivin gene. Results The frequencies of CC, CG, GG genotypes and C and G alleles in HCC cases at rs8073069 were 8.52%, 35.22%, 56.25%, 2.14%, 7.3.86% Respectively, which were 6.12%, 3.90%, 54.08%, 26.02%, 7% and 3.98% respectively. There was no significant difference in genotype and allele distribution of rs8073069 between HCC cases and control group under different genetic patterns (P > 0.05). The frequency of CC, CT, TT genotypes and C, T alleles of rs1042489 in HCC cases were 17.61%, 55.68%, 26.70%, 45.45% and 54.55% respectively in control group The frequencies of rs1042489 genotypes and alleles were not significantly different among HCC cases and controls (P> 0.05), with frequency of 13.78%, 52.04%, 34.18%, 39.80%, 60.20% ); Multivariate Logistic regression analysis showed that HBsAg + and hepatitis B history were the risk factors of HCC in additive genetic model r, s8073069G-rs1042489T haplotype was the protective factor of HCC; environmental factors and haplotypes in different genetic modes Type interaction analysis showed that the haplotype and the environment were not found in the dominant, recessive and additive genetic models Pigment interaction (P> 0.05). Conclusion The polymorphisms of rs8073069 and rs10424892 of survivin gene in Han population are not associated with the susceptibility to HCC. The rs8073069G-rs1042489T haplotype is a protective haplotype of HCC.