论文部分内容阅读
建立了一套培养和处理粘附ＬＡＫ细胞（Ａ－ＬＡＫ）的系统，将１２例缓解期急性髓系白血病（ＡＭＬ）患者（ＲＰＳ）的Ａ－ＬＡＫ细胞与常规制备ＬＡＫ细胞（ＲＴ－ＬＡＫ）进行了对照研究，结果显示ＲＰＳ－Ａ－ＬＡＫ细胞于培养第１０天时其扩增指数（２０．１±１３．９）较ＲＴ－ＬＡＫ细胞的扩增指数（７．５±２．１）明显提高（Ｐ＜０．０５）。形态学研究显示Ａ－ＬＡＫ细胞主要由大颗粒淋巴细胞组成，免疫表型分析显示其主要由ＣＤ１６＋的ＮＫ细胞组成，ＲＴ－ＬＡＫ细胞主要由ＣＤ３＋的Ｔ细胞组成。其杀伤活性与ＣＤ１６＋ＮＫ细胞之间有很好的相关性（ｒ＝０．８２，Ｐ＜０．０５）。这提示ＣＤ１６＋ＮＫ细胞是Ａ－ＬＡＫ细胞的主要组成细胞，代表了杀伤功能最强的亚群。
A set of system for culturing and processing of adherent LAK cells (A-LAK) was established. A-LAK cells from 12 patients with acute myeloid leukemia (AML) in remission (RPS) A control study showed that the proliferation index (20.1 ± 13.9) of RPS-A-LAK cells on day 10 was significantly higher than that of RT-LAK cells (7.5 ± 2.1) Increase (P <0.05). Morphological studies showed that A-LAK cells mainly consisted of large granulocytes and immunophenotype analysis showed that they mainly consisted of CD16 + NK cells. RT-LAK cells mainly consisted of CD3 + T cells. There was a good correlation between the killing activity and CD16 + NK cells (r = 0.82, P <0.05). This suggests that CD16 + NK cells are the major constitutive cells of A-LAK cells and represent the strongest subpopulation of A-LAK cells.