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目的:探讨早期氢水和有氧运动干预对心肌梗死大鼠左心室β_3-AR及其下游分子e NOS蛋白表达的影响。方法:雄性SD大鼠随机分为假手术组(S)、心肌梗死组(MI)、有氧运动+心梗组(EM)、氢水+心梗组(HM)和氢水+有氧运动+心梗组(HEM)。HM和HEM组大鼠均给予早期氢水灌胃4 m L,灌胃时间5 d/周×3周。EM和HEM组大鼠均进行早期3周递增式跑台运动。3周氢水及运动干预后MI,EM,HM和HEM组大鼠均结扎心脏左冠状动脉前降支,建立MI模型。S组大鼠实施假手术。5组大鼠术后45min测定血流动力学指标判定心功能变化。心脏Masson染色测定胶原容积百分比(CVF)。采用荧光免疫组化法检测左心室β_3-AR蛋白表达。采用western blot法检测β_3-AR及下游e NOS蛋白水平。结果:MI后心脏CVF值显著升高(P<0.01),心功能显著降低,同时左心室β_3-AR蛋白表达有增加趋势。与MI组比较,EM、HM和HEM组CVF(P<0.01)均显著降低,心功能均得到提升。EM、HM和HEM组均可见左心室β_3-AR阳性染色,同时EM和HM组β_3-AR和e NOS蛋白表达均较MI组显著增加(均P<0.05),HEM组β_3-AR和e NOS表达较MI组有增加趋势。且左心室β_3-AR与e NOS蛋白表达呈显著正相关(R=0.47,P<0.01)。结论:心梗之前进行3周氢水和运动干预可减轻心肌纤维化程度,提升心梗大鼠心功能,增加左心室β_3-AR表达,上调其下游e NOS蛋白表达。氢水联合运动干预对β_3-AR影响并不及单一干预效果。而3种干预方式对心功能提升并无差异。
AIM: To investigate the effect of early hydronephrosis and aerobic exercise on left ventricular β 3 -AR and eNOS protein expression in myocardial infarction rats. Methods: Male SD rats were randomly divided into sham operation group (S), myocardial infarction group (MI), aerobic exercise + myocardial infarction group (EM), hydrogen water + myocardial infarction group (HM) and hydrogen water + aerobic exercise + Myocardial infarction group (HEM). The rats in both HM and HEM groups were treated with early hydrogen water gavage for 4 m L and gavage time 5 d / week for 3 weeks. Rats in both EM and HEM groups were subjected to progressive treadmill exercise in the early 3 weeks. The rats in MI, EM, HM and HEM groups were ligated with left anterior descending coronary artery and the MI model was established after 3 weeks of hydronephrosis and exercise intervention. Rats in S group were sham operated. The hemodynamic parameters of the rats in each group were determined 45min after operation for changes of cardiac function. Heart Masson staining measured collagen volume percentage (CVF). The left ventricular β 3 -AR protein expression was detected by fluorescence immunohistochemistry. The β 3 -AR and downstream e NOS protein levels were detected by western blot. Results: After MI, CVF of heart increased significantly (P <0.01), heart function decreased significantly, meanwhile the expression of β 3-AR in left ventricle increased. Compared with MI group, the CVF (P <0.01) in EM, HM and HEM groups were significantly decreased, and heart function were improved. The expression of β 3 -AR and e NOS in EM and HM groups were significantly increased compared with those in MI group (all P <0.05). The levels of β 3 -AR and e NOS in HEM group were significantly higher than that in MI group The expression of MI group had an increasing trend. The left ventricular β_3-AR and eNOS protein expression was positively correlated (R = 0.47, P <0.01). Conclusion: Hydrolyzed water and exercise for 3 weeks before myocardial infarction can reduce the degree of myocardial fibrosis, improve the cardiac function, increase the expression of β 3 -AR in the left ventricle, and up-regulate the expression of e NOS protein in the heart. Hydrogen combined exercise intervention on β_3-AR is not as a single intervention effect. There was no difference between the three interventions in improving cardiac function.