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喹啉衍生物对黑色素有亲和性,可用于检测黑色素瘤,作者用~(131)I标记氯喹类似物,试验能否检测眼的黑色素瘤。用氯胺-T法标记氯喹(1)得到的标记产物收率很低,根据不同溶剂的层析分析,标记产物的组成十分复杂。不同的pH和载体碘量均影响标记产物的比例,在pH>4时,主要产物为3-碘氯喹(4),并分得具有几乎相同R_f值的3-氯氯喹(3),改用HNO_2,K_2S_2O_8和H_2O_2/乳酸过氧化酶时,产率同样很低(10～20％),且形成一种以上的标记产物。这可能由于侧链的烷胺基与I~+产生副反应或被氯胺-T,HNO_2等氧化剂氧化而使反应产物
Quinoline derivatives have affinity for melanin and can be used to detect melanoma. The authors used chloroquine analogues labeled with ~ (131) I to test whether they could detect ocular melanoma. The yield of labeled product obtained by chloramphenicol (1) labeling with chloramine-T method is very low. According to the chromatographic analysis of different solvents, the composition of the labeled product is very complicated. The different pH and carrier iodine contents all affect the ratio of labeled products. At pH> 4, the major product is 3-iodochloroquine (4), and 3-chloro chloroquine (3) with almost the same Rf value is assigned to HNO 2, K 2 S 2 O 8 and H 2 O 2 / lactate peroxidase, the yields are equally low (10-20%) and more than one labeled product is formed. This may be due to the side chain alkylamino and I ~ + side reactions or oxidants such as chloramine-T, HNO 2 oxidation so that the reaction products