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【目的】利用各种生物信息学工具分析hsa-miR-378转录调控、靶基因功能,以期为研究hsa-miR-378在人脂肪细胞分化过程中的功能与调控机制提供线索。【方法】应用UCSC Genome Browser、Promoter scan等在线工具,分析hsa-miR-378在基因组上下游10kb以内的CpG岛分布情况、转录起始位置(TSS)、转录因子结合位置(TFBS)等;选择TargetScan、PicTar、MiRanda三种计算方法预测hsa-miR-378的靶基因,取三个预测结果的交集,并合并DIANA LAB-TarBase 6.0数据库的已验证靶标,对所得靶基因集分别进行GO注释描述、GO富集分析和pathway富集分析。【结果】hsa-miR-378可能具有独立的启动子,可能受C/EBPβ转录因子的调控;其预测靶基因集合富集于转录调控、蛋白质修饰、细胞分化等生物学过程和功能(P<0.01);并显著富集于TGF-β信号通路、Wnt信号通路等4个信号通路,以及甲状腺癌、系统性红斑狼疮等8个疾病通路中(P<0.05)。【结论】通过对hsa-miR-378转录调控元件、靶基因的分析,为hsa-miR-378在人脂肪细胞中的功能与调控机制研究提供了线索和理论依据。
【Objective】 The objective of this study was to analyze the function of hsa-miR-378 in transcriptional regulation and target gene function by using various bioinformatics tools in order to provide clues for studying the function and regulation mechanism of hsa-miR-378 in human adipocyte differentiation. 【Methods】 CpG island distribution, transcriptional start site (TSS) and transcription factor binding site (TFBS) within 10kb upstream and downstream of hsa-miR-378 were analyzed by on-line tools such as UCSC Genome Browser and Promoter scan. TargetScan, PicTar and MiRanda were used to predict the target gene of hsa-miR-378. The intersection of the three predicted results was obtained and the validated target of the DIANA LAB-TarBase 6.0 database was combined. GO enrichment analysis and pathway enrichment analysis. 【Results】 hsa-miR-378 may have an independent promoter, which may be regulated by C / EBPβ transcription factor. Its target gene pool is predicted to be enriched in biological processes and functions such as transcriptional regulation, protein modification and cell differentiation (P < 0.01). It was also significantly enriched in four signaling pathways, including TGF-β signaling pathway and Wnt signaling pathway, as well as in eight pathways including thyroid cancer and systemic lupus erythematosus (P <0.05). 【Conclusion】 The analysis of hsa-miR-378 transcriptional regulatory elements and target genes provided clues and theoretical basis for the study of the function and regulation mechanism of hsa-miR-378 in human adipocytes.