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为探究薄壳山核桃“小脚”现象的导管分子特性,采用组织离析法与显微照相技术,观察以‘Mahan’品种为砧木,‘Pawnee’品种为接穗的嫁接苗与非嫁接苗(‘Mahan’)导管分子类型及大小。结果表明,与非嫁接苗相比,嫁接苗砧木根段不同尾类型导管分子数目未见变化,两端壁水平导管分子数目多38.46%;砧木茎段两端无尾导管分子数目多50.00%,砧木茎段两端壁水平导管分子数目多92.31%;砧木根段平均倾斜角度比根小36.57%、砧木茎段平均倾斜角度比茎下部小30.56%;砧木根段平均长度比根短12.62%,平均宽度比根小4.18%,砧木茎段平均长度比茎下部短3.89%,平均宽度比茎下部宽11.68%。综上所述,嫁接薄壳山核桃“小脚”现象可能与砧木根段和砧木茎段木质部导管分子的形态与大小变化有关。
In order to explore the molecular characteristics of catheters of “Hickory” “Legs ” phenomenon, tissue isolation and micrography were used to observe the effects of ’Mahan’ as rootstock and ’Pawnee’ ’Mahan’) Catheter molecule type and size. The results showed that compared with the non-grafted seedlings, the numbers of different tail-type catheters in the roots of grafted seedlings did not change, and the number of horizontal duct-like molecules in the two end walls was 38.46% more than that in the non-grafted seedlings. The average number of horizontal root segments of rootstocks was 36.57% less than that of roots and the average angle of slope of rootstocks was 30.56% less than that of stems. The average root length of rootstocks was 12.62% The average width was 4.18% less than that of roots. The average length of rootstock stems was 3.89% less than that of stems and the average width was 11.68% wider than the lower part of stems. In summary, the grafted thin walnut “small feet ” phenomenon may be related to rootstock and rootstock section of the xylem catheter morphology and size changes.