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目的：建立血清中硫酸奈替米星的高效液相色谱间接光度检测法（ＨＰＬＣＩＤＰ）。方法：以烟酰胺为检测剂，庚烷磺酸钠为反离子，固定相ＳｐｈｅｒｉｓｏｒｂＣ１８，流动相为甲醇ｐＨ２的磷酸缓冲液（２０∶８０），内含烟酰胺０．４ｍｍｏｌ·Ｌ－１，庚烷磺酸钠５ｍｏｌ·Ｌ－１。测定了４例患者单剂量ｉｍ后的血清浓度并计算药动学参数。结果：线性范围０．５ｍｇ·Ｌ－１～１５．０ｍｇ·Ｌ－１，线性回归方程ｃ＝０．８５５－４．３８４×１０－６Ｈ，ｒ＝０．９９８。回收率平均为９３．２７％，ＲＳＤ日内平均为４．９５％，日间平均为５．７１％。结论：本法操作简便，灵敏度高，适于作为血清中硫酸奈替米星的检测方法
Objective: To establish a method for the determination of netilmicin sulfate in serum by HPLC-IDP. Methods: Nicotinamide was used as the detection reagent and sodium heptane sulfonate as the counter ion. The stationary phase was Spherisorb C18. The mobile phase consisted of methanol-pH2 phosphate buffer (20:80) containing nicotinamide 0.4 mmol·L-1, Heptane sulfonate 5mol · L-1. Four patients were measured after a single dose im serum concentration and calculated pharmacokinetic parameters. Results: The linear range was 0.5 mg · L-1 ~ 15.0 mg · L-1. The linear regression equation was 0.855-4.384 × 10-6H, r = 0.998. The average recovery was 93.27%. The RSD was 4.95% on average and the average daytime was 5.71%. Conclusion: The method is simple, sensitive and suitable for the detection of netilmicin sulfate in serum