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AIM:To study the effect of cerebral ischemia-reperfusion(CI /RP)injury on the function and cycle of T cells in ra t.METHODS:Using animal model of brain ischemia-reperfusion injury,H-TdR incorporation and flow cytom eter to detect the change of T cell function a nd cell cycle.RESULTS:After CI /RP,the proliferation ability,clon e formation rate and IL-2production ability of T cells of experimental group were apparently lower than those of contr ol group(P<0.01),and it more apparent with prolonged ischemia time;with CI /RP the recovery of 3-hour gro up was more rapid than that of 6-hour group;after CI /RP injury,the proliferation cycle of T cells in each grou ps changed,the cells in G 0 /G 1 stage were more than those of control group(P<0.01);while the cells in S +G 2 +M stages were few than those of contr ol group(P<0.01);when CI /RP,amount of cells in G 0 /G 1 stage decrease gradually and that of cells in S +G 2 +M stages increase gradual-ly.CONCLUSION:after CI /RP,the function of rat sple en T cells decrease and the proliferation and different iation of cells were inhibited.Early-stage reperfusion can recover damaged immune function as soon as possible.
AIM: To study the effect of cerebral ischemia-reperfusion (CI / RP) injury on the function and cycle of T cells in rat. METHODS: Using animal model of brain ischemia-reperfusion injury, H-TdR incorporation and flow cytometry detect the change of T cell function a nd cell cycle .RESULTS: After CI / RP, the proliferation ability, clon e formation rate and IL-2 production ability of T cells of experimental group were apparently lower than those of contr ol group (P < 0.01), and it more apparent with prolonged ischemia time; with CI / RP the recovery of 3-hour gro up was more rapid than that of 6-hour group; after CI / RP injury, the proliferation cycle of T cells in each grou ps changed, the cells in G 0 / G 1 stage were more than those of control group (P <0.01); while the cells in S + G 2 + M stages were few than those of contr ol group (P <0.01); when CI / RP, amount of cells in G 0 / G 1 stage decrease gradually and that of cells in S + G 2 + M stages increase gradual-ly. CONCLUSI ON: after CI / RP, the function of rat sple en T cells decrease and the proliferation and different iation of cells were inhibited. Early-stage reperfusion can recover damaged immune function as soon as possible.