论文部分内容阅读
为评价南京地区抗草铵膦转bar基因水稻Ⅱ优86B及其恢复系86B在田间释放后抗性基因漂移到杂草稻的潜在风险,在农田生态环境下以转bar基因Ⅱ优86B和恢复系86B为花粉供体,以丹东、泰州、肇庆3地采集的杂草稻为花粉接受材料,检测抗性基因漂移率。结果表明,在杂草稻移栽和直播两种种植方式下,只有移栽方式下的泰州杂草稻和直播方式下的肇庆杂草稻与Ⅱ优86B花期相遇,检测到的基因漂移率分别为0.136%和0.018%。与恢复系86B花期相遇的只有直播方式下的肇庆杂草稻,检测到的基因漂移率为0.016%。以上研究结果表明在南京地区抗草铵膦转基因水稻Ⅱ优86B及恢复系86B向肇庆杂草稻以及Ⅱ优86B向泰州杂草稻存在抗性基因漂移的风险。
In order to evaluate the potential risk of resistance gene drifting to weedy rice (Oryza sativa L.) after the glufosinate-resistant bar transgenic rice Ⅱ You 86B and its restorer 86B in the field were released in the field in Nanjing, Line 86B is a pollen donor. The weedy rice collected from Dandong, Taizhou and Zhaoqing was pollen receiving material, and the resistance gene drift rate was detected. The results showed that only the transplanting of Taizhou weedy rice and direct seeding Zhaoqing weedy rice met the Ⅱ You 86B florescence in weedy rice transplanting and direct seeding. The detected gene drift rates were respectively 0.136% and 0.018%. Only the Zhaoqing weedy rice meeting the 86B flowering stage of restorer line had the gene drift rate of 0.016%. The above results show that there is a risk of resistance gene drift to the weedy rice of Zhaoqing and Ⅱyou86B to the weedy rice of Taizhou in ⅡYou 86B and 86B of the transgenic lines resistant to glufosinate in Nanjing.