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目的 用重组质粒ｐｃ Ｄ Ｎ Ａ３ － Ｐ３０ 免疫 Ｂ Ａ Ｌ Ｂｃ 小鼠， 观察其 Ｄ Ｎ Ａ 免疫所诱导的小鼠体液免疫反应和保护性作用。方法 大量制备质粒 Ｄ Ｎ Ａ， 肌肉注射免疫小鼠。 Ｅ Ｌ Ｉ Ｓ Ａ 测定 Ｉｇ Ｇ 抗体滴度； 免疫鼠血液组织 Ｐ３０ 基因 Ｐ Ｃ Ｒ 扩增； 弓形虫攻击感染。结果 用 Ｅ Ｌ Ｉ Ａ Ｓ 法检测的抗体 Ｉｇ Ｇ 滴度１∶２５６０ ， 免疫后３ 周及６ 个月从免疫鼠血液组织中检测到 Ｐ３０ 基因；攻击感染实验组的存活时间较对照时间延长（ Ｐ＜ ００５） ， 结论 重组质粒ｐｃ Ｄ Ｎ Ａ３ － Ｐ３０ 免 Ｂ Ａ Ｌ Ｂｃ 小鼠可诱导一定的体液免疫应答， 对抗攻击感染具有一定的保护性。
OBJECTIVE: To investigate the humoral immune response and protective effect of mice immunized with D N A against B A L Bc mice by recombinant plasmid pc D N A3 - P30. Methods Plasmid D N A was prepared in large quantities and immunized intramuscularly. E L I S A Ig G antibody titers were measured; P C R amplification of P30 gene in the blood of immunized mice; Toxoplasma gondii infection. Results The IgM titer 1:2560 was detected by ELIAS method. The P30 gene was detected from the blood of immunized mice at 3 weeks and 6 months after immunization. The survival time of experimental group was longer than that of control group P <005). Conclusions The recombinant plasmid pcDNA3 - P30 - free B L Bc mice can induce a certain humoral immune response and have some protection against attack of infection.