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胶质细胞成熟因子(GMF)是从成年牛脑中提取的一种酸性蛋白质,它能可逆地促进成星形胶质细胞形态和化学的分化。我们现在建立富含神经元的生后7天大鼠小脑皮质分离细胞原代培养物,间接免疫荧光染色分类计数证明,该原代培养物中神经元占细胞总数的97%,其中绝大部分是颗粒细胞。加纯化的 GMF 在此培养物,可明显促进神经元的生存;而不加 GMF 的对照培养物,神经元的数目明显减少。纯化 GMF 的这种作用与其剂量有关,最适的刺激剂量浓度为250 ng/ml。虽然对 GMF 影响小脑皮质神经元生存的机制尚不清楚,但实验结果提示,GMF 的功能不只限于对胶质细胞,它在中枢神经系统内,也可能是一种对神经元起神经营养作用的生长因子。
Glial cell maturation factor (GMF) is an acidic protein extracted from adult bovine brain that reversibly promotes morphological and chemical differentiation into astrocytes. We now establish neuronal-derived primary cultures of rat cerebellar cortex after 7 days of birth. Indirect immunofluorescence staining is used to classify and count the neurons in the primary culture, accounting for 97% of the total number of cells, most of which Is a granular cell. Addition of purified GMF in this culture significantly promoted the survival of neurons, while the number of neurons decreased significantly in GMF-free control cultures. This effect of GMF purification depends on its dose, with the optimal stimulation dose being 250 ng / ml. Although the mechanism by which GMF affects the survival of neurons in the cerebellar cortex is not clear, experimental results suggest that the function of GMF is not limited to glial cells, it may also be neuronal neurotrophic in the central nervous system Growth factor.