论文部分内容阅读
目的 :研究胞外调节蛋白激酶 (ERK)在糖尿病大鼠肾脏中的表达及意义 ,并探讨其激活的机制。方法 :将大鼠肾脏系膜细胞进行体外培养 ,用激光共聚焦显微镜研究高糖、血管紧张素Ⅱ (AngⅡ )对系膜细胞ERK活性的影响。用链脲佐菌素 (STZ)复制糖尿病模型 ,于 2周时处死大鼠 ,取肾进行ERK免疫组化染色 ,检测其在肾脏中的表达。结果 :体外培养的正常细胞中 ,ERK定位于胞浆 ,当AngⅡ和高糖作用于系膜细胞后 ,可使ERK从胞浆转移至胞核。糖尿病大鼠肾小球ERK表达较正常大鼠明显增强 (P <0 .0 5 )。结论 :高糖和AngⅡ可激活系膜细胞ERK ,ERK参与了糖尿病肾病的发生
Objective: To investigate the expression and significance of extracellular regulated protein kinase (ERK) in the kidney of diabetic rats and its mechanism of activation. Methods: Rat renal mesangial cells were cultured in vitro. The effect of high glucose and AngⅡ on the activity of ERK in mesangial cells was investigated by laser confocal microscopy. The diabetic model was replicated with streptozotocin (STZ). The rats were sacrificed at 2 weeks and the kidneys were harvested for ERK immunohistochemical staining to detect their expression in the kidney. Results: In normal cells cultured in vitro, ERK localized in the cytoplasm. When AngⅡ and high glucose were applied to mesangial cells, ERK could be transferred from cytoplasm to nucleus. The expression of ERK in glomeruli of diabetic rats was significantly higher than that in normal rats (P <0.05). Conclusion: High glucose and Ang Ⅱ can activate mesangial cells ERK, ERK involved in the occurrence of diabetic nephropathy