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赖氏法测转氨酶时,标准曲线中标准管酮酸量与相应的酶活力单位不呈直线关系,绘制及重校标准曲线时,手续烦琐且易造成误差。分析上述问题,根据吸光度与酶活力在坐标系中曲线的特点和各种插值法的特点,在此向读者介绍一种三次样条(spline)插值方法绘制ALT标准曲?
When the aminotransferase was measured by the Lai’s method, the standard keto acid amount in the standard curve was not in a straight line relationship with the corresponding enzyme activity unit. When drawing and recalibrating the standard curve, the procedure was cumbersome and could easily cause errors. To analyze the above problems, according to the characteristics of absorbance and enzyme activity in the coordinate system and the characteristics of various interpolation methods, the reader is introduced to a cubic spline interpolation method to draw ALT standard music.