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本研究以甘蓝型油菜GH06和中油821为亲本杂交,后代通过“一粒传法”连续自交8代构建重组自交系群体SWU-2为材料,利用本实验室已构建的遗传连锁图谱和复合区间作图法(CIM)对三个不同环境下含油量及含油量环境钝感系数进行QTL分析。结果表明:(1)不同环境中共检测到49个含油量相关QTL分别位于10个不同连锁群上,单个QTL可解释3.31%~12.47%的表型变异,42个含油量环境钝感系数QTL分别位于12个不同连锁群上,单个QTL可解释表型变异的4.05%~14.68%;(2)7个含油量相关的QTL与含油量钝感系数QTL相互重叠,分别位于第1、5、8、10、15和20连锁群上。研究结果表明明甘蓝型油菜含油量相关性状表现为多基因控制的数量性状,基因表达受环境影响较大,本研究为甘蓝型油菜含油量钝感基因的筛选提供了依据,有助于促进甘蓝型油菜高油稳油分子标记辅助育种的发展。
In this study, we crossed the parents of the Brassica napus L. GH06 and Zhongyou 821, and the offspring was crossed with the SWU-2 inbred lines of inbred lines for 8 generations through " Map and composite interval mapping (CIM) were used to analyze the QTLs of the environmental sensitivity of oil content and oil content in three different environments. The results showed that: (1) A total of 49 oil-related QTLs detected in different environments were located in 10 different linkage groups. A single QTL explained 3.31% ~ 12.47% of the phenotypic variation, 42 QTLs for environmental oil sensitivity Located in 12 different linkage groups. A single QTL explained 4.05% ~ 14.68% of the phenotypic variance. (2) Seven oil-related QTLs and oil-content sensitive QTLs overlapped each other, , 10, 15 and 20 on the chain group. The results showed that oil content-related traits in Brassica napus showed quantitative traits controlled by multiple genes, and gene expression was greatly affected by the environment. This study provided the basis for selection of oil-susceptible genes in Brassica napus and contributed to the promotion of cabbage Development of high oil stable oil molecular marker assisted breeding of rape.