Use of arrays to investigate the contribution of ATP-binding cassette transporters to drug resistanc

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Multidrug resistance(MDR)is a major problem in cancer chemotherapy.One of the best known mechanisms of MDRis the elevated expression of ATP-binding cassette(ABC)transporters.While some members of human ABC transportershave been shown to cause drug resistance with elevated expression,it is not yet known whether the over-expression ofother members could also contribute to drug resistance in many model cancer cell lines and clinics.The recent develop-ment of microarrays and quantitative PCR arrays for expression profiling analysis of ABC transporters has helped addressthese issues.In this article,various arrays with limited or full list of ABC transporter genes and their use in identifyingABC transporter genes in drug resistance and chemo-sensitivity prediction will be reviewed. Multidrug resistance (MDR) is a major problem in cancer chemotherapy. One of the best known mechanisms of MDRis the elevated expression of ATP-binding cassette (ABC) transporters. Whilst some members of human ABC transporters have been shown to cause drug resistance with elevated expression , it is not yet known whether the over-expression ofother members could also contribute to drug resistance in many model cancer cell lines and clinics.The recent develop-ment of microarrays and quantitative PCR arrays for expression profiling analysis of ABC transporters has helped addresse issues .In this article, various arrays with limited or full list of ABC transporter genes and their use in identifyingABC transporter genes in drug resistance and chemo-sensitivity prediction will be reviewed.
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